Tag Archives: Synthetic Biology

Applied Biosystems 3400 DNA Synthesizer

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Applied Biosystems 3400 DNA Synthesizer

The Applied Biosystems 3400 DNA Synthesizer is a versatile, four-column,
benchtop instrument designed for 40-nmol, 200-nmol, and 1-μmol synthesis
scales. Simultaneous synthesis and cleavage of four oligonucleotides (25-
mer at 40- or 200-nmol scales) requires approximately four hours

DNA Purity and Quality Established phosphoramidite chemistry
produces DNA that is pure and chemically authentic. The instrument routinely
synthesizes oligonucleotides with average coupling yields > 98% as measured by
HPLC analysis. A simple desalting of crude deprotected oligonucleotides is adequate for most applications

Applied Biosystems 394 DNA/RNA Synthesizer

The Applied BioSystems 394 DNA/RNA Synthesizer, automates all steps of single stranded oligonucleotide synthesis. When used as a system including Applied Biosystems reagents and columns, these instruments produce the highest quality synthetic DNA currently attainable while minimizing synthesis time and cost.

Applied Biosystems 394 DNA/RNA Synthesizer
Applied Biosystems 394 DNA/RNA Synthesizer


ABI 3400 DNA/ RNA Synthesizer

eABI 3400 DNA/ RNA Synthesizer
Applied Biosystems 3400 DNA / RNA Synthesizer
The Applied Biosystems 3400 DNA Synthesizer is a fully programmable instrument that provides four-column simultaneous synthesis, and features automatic base dilution and analysis of coupling efficiency. Fast cycle times, online cleavage, and proven phosphoramidite chemistry result in exceptionally pure oligonucleotides. Versatile, dependable, and easy to use, the 3400 synthesizer is a compact, bench-top instrument designed for 40 nmol, 200 nmol, and 1 µmol synthesis scales. Simultaneous synthesis and cleavage of four oligonucleotides (25- mer at 40- or 200-nmol scales) requires approximately four hours.


Note: The technology is very CHEAP for a nation state or corporation. The above models can be bought for under $50K. There are DIY efforts to make this even cheaper, think along the lines of 3D-printing. This clearly shows the routine competency regarding DNA/RNA synthesis and tinkering.

Intrexon Corporation – Synthetic Biology


“The ability to create and modify ‘organic’ materials on increasingly larger scales has occurred with a number of breakthroughs in genetic engineering including automated DNA sequencing, DNA synthesis, the advent of computational bioinformatics, and the creation of genetically modified organisms. Today, Intrexon’s expertise in designing DNA and constructing complex gene programs utilizing a modular, scalable approach with an embedded capacity to predict the viability of a biological solution, places it at the leading-edge of this growing synthetic biology discipline.”

RT: Genetically engineered mosquitoes battle Zika virus in Brazil


The ‘Friendly Aedes aegypti Project’ conducted by the company’s subsidiary Oxitec aims to tackle the virus spread in the city of Piracicaba, based on the success of previous preventative measures introduced across Central and South America.

“As the principal source for the fastest growing vector-borne infection in the world in Dengue Fever, as well as the increasingly challenging Zika virus, controlling the Aedes aegypti population provides the best defense against these serious diseases for which there are no cures,” said Oxitec CEO Hadyn Parry.
…Oxitec is initiating a new mosquito production facility in Piracicaba that will have capacity to protect over 300,000 people,”

…Oxitec’s previous trials on mosquito population began in April 2015 after Brazil’s National Biosafety Committee (CTNBio) gave the green light for the UK company to release the insects into the wild.

“By the end of the calendar year, results had already indicated a reduction in wild mosquito larvae by 82 percent. Oxitec’s efficacy trials across Brazil, Panama and the Cayman Islands all resulted in a greater than 90 percent suppression of the wild Ae. aegypti mosquito population – an unprecedented level of control,” the statement said.

Marketwatch Oxitec aricle

“Intrexon Corporation [http://www.dna.com/] , a leader in synthetic biology, today announced its subsidiary Oxitec and Piracicaba City Hall have expanded the ‘Friendly Aedes aegypti Project’ in Piracicaba, Brazil following strong results for controlling the Ae. aegypti mosquito population, the primary vector for dengue, chikungunya and Zika virus outbreaks around the world

Samuel Broder, M.D., SVP and Head of Intrexon’s Health Sector commented, “As a vector that transmits a number of serious diseases, the Aedes aegypti mosquito poses a major threat to public health and the economic welfare of nations. Brazil has been hard hit by dengue and the situation there has been aggravated by the recent introduction of Zika virus infections leading to a startling increase in the number of children being born with microcephaly.” Dr. Broder continued, “Through the responsible engineering of biology, we demonstrate a new paradigm of species-specific vector control resulting in dramatic reductions of dangerous mosquitoes, without persistence or harm to the ecosystem, representing a major scientific, environmental and clinical advance.”