Tag Archives: DNA/RNA Synthesis

A low-cost, open-source DIY DNA Synthesizer Project

PDF : DIY DNA Synthesizer Project

“Biology is largely about the manipulation, maintenance and transport of DNA.

Oligonucleotide synthesis is too complex and tiresome to make genomes by hand

Technical feasability has been proven by already existing commercial technologies, as well as next-generation  platforms from academia.

Material costs of these devices – under $5,000. Traditional synthesizers could be built cheaper.

Mail-order DNA synthesis companies have constructed a shared “harmful sequences” database.”

 

Applied Biosystems 3400 DNA Synthesizer

Link to PDF

Applied Biosystems 3400 DNA Synthesizer

The Applied Biosystems 3400 DNA Synthesizer is a versatile, four-column,
benchtop instrument designed for 40-nmol, 200-nmol, and 1-μmol synthesis
scales. Simultaneous synthesis and cleavage of four oligonucleotides (25-
mer at 40- or 200-nmol scales) requires approximately four hours

DNA Purity and Quality Established phosphoramidite chemistry
produces DNA that is pure and chemically authentic. The instrument routinely
synthesizes oligonucleotides with average coupling yields > 98% as measured by
HPLC analysis. A simple desalting of crude deprotected oligonucleotides is adequate for most applications

Applied Biosystems 394 DNA/RNA Synthesizer

The Applied BioSystems 394 DNA/RNA Synthesizer, automates all steps of single stranded oligonucleotide synthesis. When used as a system including Applied Biosystems reagents and columns, these instruments produce the highest quality synthetic DNA currently attainable while minimizing synthesis time and cost.

Applied Biosystems 394 DNA/RNA Synthesizer
Applied Biosystems 394 DNA/RNA Synthesizer

http://www.labx.com/item/applied-biosystems-394-dna-rna-synthesizer/LV34222695

ABI 3400 DNA/ RNA Synthesizer

eABI 3400 DNA/ RNA Synthesizer
Applied Biosystems 3400 DNA / RNA Synthesizer
The Applied Biosystems 3400 DNA Synthesizer is a fully programmable instrument that provides four-column simultaneous synthesis, and features automatic base dilution and analysis of coupling efficiency. Fast cycle times, online cleavage, and proven phosphoramidite chemistry result in exceptionally pure oligonucleotides. Versatile, dependable, and easy to use, the 3400 synthesizer is a compact, bench-top instrument designed for 40 nmol, 200 nmol, and 1 µmol synthesis scales. Simultaneous synthesis and cleavage of four oligonucleotides (25- mer at 40- or 200-nmol scales) requires approximately four hours.

http://www.bluelionbio.com/labequip.phtml?lab_equip_id=599&mode=detail&category_id=20&search_text=

Note: The technology is very CHEAP for a nation state or corporation. The above models can be bought for under $50K. There are DIY efforts to make this even cheaper, think along the lines of 3D-printing. This clearly shows the routine competency regarding DNA/RNA synthesis and tinkering.